Dissecting effectivity of a 5′ fast amplification of cDNA ends

November 6, 2020
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Dissecting effectivity of a 5′ fast amplification of cDNA ends (5′-RACE) strategy for profiling T-cell receptor beta repertoire

  • Deep sequencing of T-cell receptor (TCR) genes is highly effective at profiling immune repertoire. To organize a TCR sequencing library, multiplex polymerase chain response (mPCR) is broadly utilized and is extremely environment friendly.

 

  • That’s, most mPCR merchandise include the area vital for antigen recognition, which additionally signifies common V(D)J recombination. Multiplex PCR, nonetheless, could endure from primer bias. A promising different is 5′-RACE, which avoids primer bias by making use of just one primer pair. In 5′-RACE information, nonetheless, non-regular V(D)J recombination (e.g., TCR sequences and not using a V gene section) has been noticed and the frequency varies (30-80%) between research.

 

  • This implies that the reason for or find out how to scale back non-regular TCR sequences isn’t but well-known by the science neighborhood. Though it’s doable to take a position the trigger by evaluating the 5′-RACE protocols, cautious experimental affirmation is required and such a scientific examine continues to be not obtainable.

 

  • Right here, we examined the 5′-RACE protocol of a industrial equipment and demonstrated how a modification elevated the fraction of normal TCR-β sequences to >85%. We additionally discovered a robust linear correlation between the fraction of quick DNA fragments and the proportion of non-regular TCR-β sequences, indicating that the presence of quick DNA fragments within the library was the primary reason behind non-regular TCR-β sequences.

 

  • Subsequently, thorough elimination of quick DNA fragments from a 5′-RACE library is the important thing to excessive information effectivity. We extremely advocate conducting a fraction size evaluation earlier than sequencing, and the fraction of quick DNA fragments can be utilized to estimate the proportion of non-regular TCR sequences. As deep sequencing of TCR genes continues to be comparatively costly, good high quality management must be worthwhile.
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Analysis notice: Massive gene household of phosphoenolpyruvate carboxylase within the crassulacean acid metabolism plant Kalanchoepinnata (Crassulaceae) characterised by partial cDNA sequence evaluation

Clones coding for a 1100-bp cDNA sequence of phosphoenolpyruvate carboxylase (PEPC) of the constitutive crassulacean acid metabolism (CAM) plant Kalanchoepinnata (Lam.) Pers., have been remoted by reverse transcription-polymerase chain response (RT-PCR) and characterised by restriction fragment size polymorphism evaluation and DNA sequencing.

 

Seven distinct PEPC isogenes have been recovered, 4 in leaves and three in roots (EMBL accession numbers: AJ344052-AJ344058). Sequence similarity comparisons and distance neighbour-joining calculations separate the seven PEPC isoforms into two clades, one in every of which comprises the three PEPCs present in roots. The second clade comprises the 4 isoforms present in leaves and is split into two branches, one in every of which comprises two PEPCs most related with described beforehand CAM isoforms.

 

Of those two isoforms, nonetheless, just one exhibited considerable expression in CAM-performing leaves, however not in very younger leaves, which don’t exhibit CAM, suggesting this isoform encodes a CAM-specific PEPC. Protein sequence calculations counsel that every one isogenes are seemingly derived from a standard ancestor gene, presumably by serial gene duplication occasions. To our data, that is essentially the most complete identification of a PEPC gene household from a CAM plant, and the best variety of PEPC isogenes reported for any vascular plant up to now.

 

 

Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresiacoarctata

The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis is very delicate to salt stress and proteins concerned on this course of could also be an vital determinant of salt tolerance.

 

The halophytic plant, PorteresiacoarctataTateoka, is an in depth relative of Oryzasativa L., and has the power to face up to sudden adjustments within the soil salinity. The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt therapy (150 mm NaCl). An expression examine confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.

 

This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol therapies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts would possibly kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.

Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics

 

Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses.
In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%). The single identified virus among controls was EBV (7.69%).
DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR.
In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens.

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WDR76 cDNA Clone
MBS1267434-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR44 cDNA Clone
MBS1267496-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 600

WDR44 cDNA Clone
MBS1267496-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2655

WDR49 cDNA Clone
MBS1270547-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR49 cDNA Clone
MBS1270547-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR18 cDNA Clone
MBS1270831-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR18 cDNA Clone
MBS1270831-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR37 cDNA Clone
MBS1271703-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR37 cDNA Clone
MBS1271703-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR74 cDNA Clone
MBS1271879-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR74 cDNA Clone
MBS1271879-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR18 cDNA Clone
MBS1271880-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR18 cDNA Clone
MBS1271880-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR60 cDNA Clone
MBS1272194-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1155

WDR60 cDNA Clone
MBS1272194-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5150

WDR66 cDNA Clone
MBS1273393-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 880

WDR66 cDNA Clone
MBS1273393-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3910

WDR47 cDNA Clone
MBS1273453-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

WDR47 cDNA Clone
MBS1273453-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

WDR54 cDNA Clone
MBS1273667-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR54 cDNA Clone
MBS1273667-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR24 cDNA Clone
MBS1273900-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 745

WDR24 cDNA Clone
MBS1273900-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3300

WDR46 cDNA Clone
MBS1274239-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 590

WDR46 cDNA Clone
MBS1274239-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2605

WDR41 cDNA Clone
MBS1274442-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR41 cDNA Clone
MBS1274442-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR91 cDNA Clone
MBS1274578-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR91 cDNA Clone
MBS1274578-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR34 cDNA Clone
MBS1274709-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR34 cDNA Clone
MBS1274709-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR85 cDNA Clone
MBS1274748-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR85 cDNA Clone
MBS1274748-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR13 cDNA Clone
MBS1275052-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR13 cDNA Clone
MBS1275052-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR65 cDNA Clone
MBS1275851-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 435

WDR65 cDNA Clone
MBS1275851-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1910

WDR53 cDNA Clone
MBS1276140-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR53 cDNA Clone
MBS1276140-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR90 cDNA Clone
MBS1276163-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 315

WDR90 cDNA Clone
MBS1276163-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1385

WDR73 cDNA Clone
MBS1277186-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 385

WDR73 cDNA Clone
MBS1277186-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1690

WDR76 cDNA Clone
MBS1277238-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR76 cDNA Clone
MBS1277238-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR19 cDNA Clone
MBS1277262-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR19 cDNA Clone
MBS1277262-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR85 cDNA Clone
MBS1277295-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR85 cDNA Clone
MBS1277295-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR67 cDNA Clone
MBS1277315-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR67 cDNA Clone
MBS1277315-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR75 cDNA Clone
MBS1277561-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 780

WDR75 cDNA Clone
MBS1277561-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3455

WDR20 cDNA Clone
MBS1277815-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR20 cDNA Clone
MBS1277815-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR89 cDNA Clone
MBS1277972-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR89 cDNA Clone
MBS1277972-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR61 cDNA Clone
MBS1278250-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR61 cDNA Clone
MBS1278250-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR62 cDNA Clone
MBS1278452-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR62 cDNA Clone
MBS1278452-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR41 cDNA Clone
MBS1278785-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 350

WDR41 cDNA Clone
MBS1278785-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1525

WDR20 cDNA Clone
MBS1271465-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR20 cDNA Clone
MBS1271465-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR63 cDNA Clone
MBS1271597-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 520

WDR63 cDNA Clone
MBS1271597-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 2295

Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514)
MR204616 10 µg Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514)
MR204616L3 10 µg Ask for price

WDR45L cDNA Clone
MBS1268101-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR45L cDNA Clone
MBS1268101-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR6 cDNA Clone
MBS1267661-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1210

WDR6 cDNA Clone
MBS1267661-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 5405

WDR6 cDNA Clone
MBS1268803-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR6 cDNA Clone
MBS1268803-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR4 cDNA Clone
MBS1269790-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR4 cDNA Clone
MBS1269790-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR1 cDNA Clone
MBS1267204-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR1 cDNA Clone
MBS1267204-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR3 cDNA Clone
MBS1272968-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 160

WDR3 cDNA Clone
MBS1272968-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 670

WDR1 cDNA Clone
MBS1273887-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 330

WDR1 cDNA Clone
MBS1273887-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 1430

WDR7 cDNA Clone
MBS1274012-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 765

WDR7 cDNA Clone
MBS1274012-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 3400

WDR5 cDNA Clone
MBS1275747-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR5 cDNA Clone
MBS1275747-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

WDR4 cDNA Clone
MBS1276184-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 200

WDR4 cDNA Clone
MBS1276184-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 855

Lenti ORF particles, Wdr78 (GFP-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL
MR204616L4V 200 µl Ask for price

Lenti ORF particles, Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (cDNA clone MGC:31722 IMAGE:4920514), 200ul, >10^7 TU/mL
MR204616L3V 200 µl Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged) - Mouse WD repeat domain 78 (Wdr78)
MR209396L4 10 µg Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged) - Mouse WD repeat domain 78 (Wdr78)
MR209396L3 10 µg Ask for price

Lenti ORF clone of Wdr78 (mGFP-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)
RR208219L4 10 µg Ask for price

Lenti ORF clone of Wdr78 (Myc-DDK-tagged ORF) - Rat WD repeat domain 78 (Wdr78), (10 ug)
RR208219L3 10 µg Ask for price

Lenti-ORF clone of WDR78 (mGFP-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2
RC221613L4 10 µg Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422L4 10 µg Ask for price

Lenti ORF clone of Wdr62 (mGFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595L4 10 µg Ask for price

Lenti ORF clone of Wdr73 (mGFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438L4 10 µg Ask for price

Lenti ORF clone of Wdr48 (mGFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851L4 10 µg Ask for price

Lenti ORF clone of Wdr20a (mGFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616L4 10 µg Ask for price

Lenti ORF clone of Wdr42a (mGFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846L4 10 µg Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422L3 10 µg Ask for price

Lenti ORF clone of Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595L3 10 µg Ask for price

Lenti ORF clone of Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438L3 10 µg Ask for price

Lenti ORF clone of Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851L3 10 µg Ask for price

Lenti-ORF clone of WDR78 (Myc-DDK-tagged)-Human WD repeat domain 78 (WDR78), transcript variant 2
RC221613L3 10 µg Ask for price

Lenti ORF clone of Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616L3 10 µg Ask for price

Lenti ORF clone of Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846L3 10 µg Ask for price

WDR3 (untagged)-Human cDNA clone MGC:61946 IMAGE:6745281, complete cds
SC126422 10 µg Ask for price

Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MG209851 10 µg Ask for price

Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MG203438 10 µg Ask for price

Wdr32 (GFP-tagged) - Mouse WD repeat domain 32 (cDNA clone MGC:11993 IMAGE:3601889)
MG206473 10 µg Ask for price

Wdr36 (GFP-tagged) - Mouse WD repeat domain 36 (cDNA clone MGC:103340 IMAGE:5068941)
MG211095 10 µg Ask for price

Wdr47 (GFP-tagged) - Mouse WD repeat domain 47 (cDNA clone MGC:8037 IMAGE:3586908)
MG211187 10 µg Ask for price

Wdr59 (GFP-tagged) - Mouse WD repeat domain 59 (cDNA clone MGC:91308 IMAGE:30355918)
MG211357 10 µg Ask for price

Wdr67 (GFP-tagged) - Mouse WD repeat domain 67 (cDNA clone MGC:60990 IMAGE:30016266)
MG211408 10 µg Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MG211422 10 µg Ask for price

Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MG211595 10 µg Ask for price

Wdr37 (untagged) - Mouse WD repeat domain 37 (cDNA clone MGC:58091 IMAGE:6817089), (10ug)
MC206152 10 µg Ask for price

Wdr77 (untagged) - Mouse WD repeat domain 77 (cDNA clone MGC:6890 IMAGE:2654151), (10ug)
MC200277 10 µg Ask for price

Wdr48 (untagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), (10ug)
MC217839 10 µg Ask for price

Wdr73 (untagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), (10ug)
MC218435 10 µg Ask for price

Wdr24 (untagged) - Mouse WD repeat domain 24 (cDNA clone MGC:47001 IMAGE:5148210), (10ug)
MC206644 10 µg Ask for price

Wdr68 (untagged) - Mouse WD repeat domain 68 (cDNA clone MGC:58053 IMAGE:6409531), (10ug)
MC206690 10 µg Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), (10ug)
MC223031 10 µg Ask for price

Wdr62 (untagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), (10ug)
MC223128 10 µg Ask for price

Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MG208616 10 µg Ask for price

Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MG204846 10 µg Ask for price

WDR78 cloning plasmid
CSB-CL719620HU1-10ug 10ug
EUR 640.8
Description: A cloning plasmid for the WDR78 gene.

WDR78 cloning plasmid
CSB-CL719620HU2-10ug 10ug
EUR 986.4
Description: A cloning plasmid for the WDR78 gene.

Wdr20a (untagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), (10ug)
MC217866 10 µg Ask for price

Wdr42a (untagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38511 IMAGE:5352941), (10ug)
MC206626 10 µg Ask for price

Wdr40b (untagged) - Mouse WD repeat domain 40B (cDNA clone MGC:76797 IMAGE:30431243), (10ug)
MC206734 10 µg Ask for price

Lenti ORF clone of Wdr4 (mGFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503L4 10 µg Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055)
MR211422 10 µg Ask for price

Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269)
MR211595 10 µg Ask for price

Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614)
MR203438 10 µg Ask for price

Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339)
MR209851 10 µg Ask for price

Lenti ORF clone of Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503L3 10 µg Ask for price

Wdr20a (Myc-DDK-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717)
MR208616 10 µg Ask for price

Wdr42a (Myc-DDK-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701)
MR204846 10 µg Ask for price

WDR37 (untagged)-Human WD repeat domain 37 (cDNA clone MGC:26330 IMAGE:4794184), complete cds
SC123593 10 µg Ask for price

Wdr4 (GFP-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MG206503 10 µg Ask for price

Wdr8 (untagged) - Mouse WD repeat domain 8 (cDNA clone MGC:28791 IMAGE:4501325), (10ug)
MC218347 10 µg Ask for price

WDR59 (untagged)-Human hypothetical protein FLJ12270 (cDNA clone MGC:11230 IMAGE:3938206), complete cds
SC122523 10 µg Ask for price

WDR77 (untagged)-Human methylosome protein 50, mRNA (cDNA clone MGC:4346 IMAGE:2822173), complete cds
SC126139 10 µg Ask for price

Wdr4 (Myc-DDK-tagged) - Mouse WD repeat domain 4 (cDNA clone MGC:35877 IMAGE:5027600)
MR206503 10 µg Ask for price

WDR78
CSB-CL719620HU1 10 μg plasmid + 200μl Glycerol Ask for price

WDR78
CSB-CL719620HU2 10 μg plasmid + 200μl Glycerol Ask for price

Wdr78/ Rat Wdr78 ELISA Kit
ELI-16965r 96 Tests
EUR 1063.2

WDR25 (untagged)-Human pre-mRNA splicing factor-like (cDNA clone MGC:4645 IMAGE:3529568), complete cds
SC122376 10 µg Ask for price

WDR85 (untagged)-Human chromosome 9 open reading frame 112, mRNA (cDNA clone MGC:3209 IMAGE:3502107), complete cds
SC126100 10 µg Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL
MR211422L4V 200 µl Ask for price

Lenti ORF particles, Wdr62 (GFP-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL
MR211595L4V 200 µl Ask for price

Lenti ORF particles, Wdr73 (GFP-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL
MR203438L4V 200 µl Ask for price

Lenti ORF particles, Wdr48 (GFP-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL
MR209851L4V 200 µl Ask for price

Lenti ORF particles, Wdr20a (GFP-tagged) - Mouse WD repeat domain 20a (cDNA clone MGC:30262 IMAGE:4221717), 200ul, >10^7 TU/mL
MR208616L4V 200 µl Ask for price

Lenti ORF particles, Wdr42a (GFP-tagged) - Mouse WD repeat domain 42A (cDNA clone MGC:38390 IMAGE:5345701), 200ul, >10^7 TU/mL
MR204846L4V 200 µl Ask for price

WDR78 siRNA
20-abx939775
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

WDR78 siRNA
20-abx939776
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:67271 IMAGE:6817055), 200ul, >10^7 TU/mL
MR211422L3V 200 µl Ask for price

Lenti ORF particles, Wdr62 (Myc-DDK-tagged) - Mouse WD repeat domain 62 (cDNA clone MGC:65624 IMAGE:6405269), 200ul, >10^7 TU/mL
MR211595L3V 200 µl Ask for price

Lenti ORF particles, Wdr73 (Myc-DDK-tagged) - Mouse WD repeat domain 73 (cDNA clone MGC:28423 IMAGE:4037614), 200ul, >10^7 TU/mL
MR203438L3V 200 µl Ask for price

Lenti ORF particles, Wdr48 (Myc-DDK-tagged) - Mouse WD repeat domain 48 (cDNA clone MGC:61205 IMAGE:5698339), 200ul, >10^7 TU/mL
MR209851L3V 200 µl Ask for price

WDR78 Antibody
E306990 100ug/200ul
EUR 275
Description: Available in various conjugation types.

WDR78 Antibody
MBS8527115-01mg 0.1mg
EUR 345
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