Dissecting effectivity of a 5′ fast amplification of cDNA ends

November 6, 2020
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Dissecting effectivity of a 5′ fast amplification of cDNA ends (5′-RACE) strategy for profiling T-cell receptor beta repertoire

  • Deep sequencing of T-cell receptor (TCR) genes is highly effective at profiling immune repertoire. To organize a TCR sequencing library, multiplex polymerase chain response (mPCR) is broadly utilized and is extremely environment friendly.

 

  • That’s, most mPCR merchandise include the area vital for antigen recognition, which additionally signifies common V(D)J recombination. Multiplex PCR, nonetheless, could endure from primer bias. A promising different is 5′-RACE, which avoids primer bias by making use of just one primer pair. In 5′-RACE information, nonetheless, non-regular V(D)J recombination (e.g., TCR sequences and not using a V gene section) has been noticed and the frequency varies (30-80%) between research.

 

  • This implies that the reason for or find out how to scale back non-regular TCR sequences isn’t but well-known by the science neighborhood. Though it’s doable to take a position the trigger by evaluating the 5′-RACE protocols, cautious experimental affirmation is required and such a scientific examine continues to be not obtainable.

 

  • Right here, we examined the 5′-RACE protocol of a industrial equipment and demonstrated how a modification elevated the fraction of normal TCR-β sequences to >85%. We additionally discovered a robust linear correlation between the fraction of quick DNA fragments and the proportion of non-regular TCR-β sequences, indicating that the presence of quick DNA fragments within the library was the primary reason behind non-regular TCR-β sequences.

 

  • Subsequently, thorough elimination of quick DNA fragments from a 5′-RACE library is the important thing to excessive information effectivity. We extremely advocate conducting a fraction size evaluation earlier than sequencing, and the fraction of quick DNA fragments can be utilized to estimate the proportion of non-regular TCR sequences. As deep sequencing of TCR genes continues to be comparatively costly, good high quality management must be worthwhile.
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YF-PA17860 50 ug
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NUDCD3 cloning plasmid
CSB-CL811605HU1-10ug 10ug
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Description: A cloning plasmid for the NUDCD3 gene.

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Description: A cloning plasmid for the NUDCD3 gene.

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FNab05897 100µg
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Description: Antibody raised against NUDCD3

NUDCD3 Blocking Peptide
33R-4441 100 ug
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Description: A synthetic peptide for use as a blocking control in assays to test for specificity of NUDCD3 antibody, catalog no. 70R-3285

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NUDCD3 Antibody, HRP conjugated
1-CSB-PA811605LB01HU
  • EUR 380.40
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  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

NUDCD3 Antibody, FITC conjugated
1-CSB-PA811605LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

NUDCD3 Antibody, Biotin conjugated
1-CSB-PA811605LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against NUDCD3. Recognizes NUDCD3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

NUDCD3 ELISA KIT|Human
EF001364 96 Tests
EUR 826.8

NUDCD3 Recombinant Protein (Human)
RP021817 100 ug Ask for price

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RP021820 100 ug Ask for price

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RP214763 100 ug Ask for price

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RP155360 100 ug Ask for price

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ORF071589 1.0 ug DNA
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ORF007274 1.0 ug DNA
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ORF051788 1.0 ug DNA
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NUDCD3 Polyclonal Antibody, Biotin Conjugated
A60091-050 50 ul
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A60091-100 100 ul
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NUDCD3 Polyclonal Antibody, FITC Conjugated
A60092-050 50 ul
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A60092-100 100 ul
EUR 423.5

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A60093-050 50 ul
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A60093-100 100 ul
EUR 423.5

NUDCD3 Polyclonal Antibody, Biotin Conjugated
A60091
  • EUR 684.66
  • EUR 302.50
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NUDCD3 Polyclonal Antibody, FITC Conjugated
A60092
  • EUR 684.66
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NUDCD3 Polyclonal Antibody, HRP Conjugated
A60093
  • EUR 684.66
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cDNA Synthesis SuperMix
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cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310 40 reactions
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

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C1634330 40 reactions
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

NudC Domain Containing 3 (NUDCD3) Antibody
20-abx302663
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NudC Domain Containing 3 (NUDCD3) Antibody
abx235897-100ug 100 ug
EUR 661.2

NUDCD3 sgRNA CRISPR Lentivector set (Human)
K1463701 3 x 1.0 ug
EUR 406.8

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K4846501 3 x 1.0 ug
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K7522401 3 x 1.0 ug
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cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
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cDNA from Arteriosclerosis: Aorta
C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

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EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

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20-abx308546
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

NudC Domain Containing 3 (NUDCD3) Antibody (FITC)
20-abx308547
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
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NudC Domain Containing 3 (NUDCD3) Antibody (Biotin)
20-abx308548
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
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  • 200 ug
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Analysis notice: Massive gene household of phosphoenolpyruvate carboxylase within the crassulacean acid metabolism plant Kalanchoepinnata (Crassulaceae) characterised by partial cDNA sequence evaluation

Clones coding for a 1100-bp cDNA sequence of phosphoenolpyruvate carboxylase (PEPC) of the constitutive crassulacean acid metabolism (CAM) plant Kalanchoepinnata (Lam.) Pers., have been remoted by reverse transcription-polymerase chain response (RT-PCR) and characterised by restriction fragment size polymorphism evaluation and DNA sequencing.

 

Seven distinct PEPC isogenes have been recovered, 4 in leaves and three in roots (EMBL accession numbers: AJ344052-AJ344058). Sequence similarity comparisons and distance neighbour-joining calculations separate the seven PEPC isoforms into two clades, one in every of which comprises the three PEPCs present in roots. The second clade comprises the 4 isoforms present in leaves and is split into two branches, one in every of which comprises two PEPCs most related with described beforehand CAM isoforms.

 

Of those two isoforms, nonetheless, just one exhibited considerable expression in CAM-performing leaves, however not in very younger leaves, which don’t exhibit CAM, suggesting this isoform encodes a CAM-specific PEPC. Protein sequence calculations counsel that every one isogenes are seemingly derived from a standard ancestor gene, presumably by serial gene duplication occasions. To our data, that is essentially the most complete identification of a PEPC gene household from a CAM plant, and the best variety of PEPC isogenes reported for any vascular plant up to now.

 

 

Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresiacoarctata

The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis is very delicate to salt stress and proteins concerned on this course of could also be an vital determinant of salt tolerance.

 

The halophytic plant, PorteresiacoarctataTateoka, is an in depth relative of Oryzasativa L., and has the power to face up to sudden adjustments within the soil salinity. The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt therapy (150 mm NaCl). An expression examine confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.

 

This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol therapies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts would possibly kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.

Search for viral agents in cerebrospinal fluid in patients with multiple sclerosis using real-time PCR and metagenomics

 

Multiple sclerosis (MS) is a chronic, immune-mediated demyelinating disease of the central nervous system of unclear etiology, but there is some evidence that viral infections could be responsible for triggering autoimmune mechanisms against myelin. We searched for viral RNA and DNA in cerebrospinal fluid (CSF) of 34 MS patients and 13 controls using RT-PCR/PCR against common neurotropic viruses.
In addition, shotgun DNA- and RNA-based metagenomics were done in 13 MS patients and 4 controls. Specific quantitative real-time RT-PCR/PCR testing revealed the presence of viral nucleic acid in seven (20.59%) MS patients and in one (7.69%) control patient. In MS patients the most frequently detected was human herpesvirus type 6 (HHV-6; 3 cases; 8.82%); followed by Epstein-Barr virus (EBV; 2 cases; 5.88%), varicella zoster virus (VZV; 1 case; 2.94%) and Enterovirus (EV; 1 case; 2.94%). The single identified virus among controls was EBV (7.69%).
DNA and RNA metagenomic assays did not identify any known eukaryotic viruses even though three of the analyzed samples were low-level positive by specific quantitative real-time PCR.
In conclusion, we detected the presence of Herpesviridae and occasionally Enteroviridae in CSF from patients with MS but their prevalence was not significantly higher than among controls. Metagenomic analysis seems to be less sensitive than real-time RT-PCR/PCR and it did not detect any potential viral pathogens.

WDR78 cloning plasmid
CSB-CL719620HU2-10ug 10ug
EUR 986.4
Description: A cloning plasmid for the WDR78 gene.

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11
MASF-5A-Clone-10E11 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10E11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5
MASF-5A-Clone-10G5 1 mg
EUR 667
Description:

Anti-ASFV P72 Antibody | MASF-5A-Clone #10G5 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Genorise® Human GLUT1 cDNA Clone, 10 ug
GR154002 10 µg
EUR 183.6

Genorise® Human cTnT cDNA Clone, 10 ug
GR154003 10 µg
EUR 183.6

Genorise® Human TNN3 cDNA Clone, 10 ug
GR154004 10 µg
EUR 183.6

Genorise® Human BNP cDNA Clone, 10 ug
GR154005 10 µg
EUR 183.6

Genorise® Human Myoglobin cDNA Clone, 10 ug
GR154006 10 µg
EUR 183.6

Genorise® Human ANP cDNA Clone, 10 ug
GR154008 10 µg
EUR 183.6

Genorise® Human Myeloperoxidase cDNA Clone, 10 ug
GR154009 10 µg
EUR 183.6

Genorise® Human GPBB cDNA Clone, 10 ug
GR154010 10 µg
EUR 183.6

Genorise® Human CRP cDNA Clone, 10 ug
GR154011 10 µg
EUR 183.6

Genorise® Human MyBPC3 cDNA Clone, 10 ug
GR154012 10 µg
EUR 183.6

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11
MALV-30A-Clone-3B11 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#3B11 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4
MALV-30A-Clone-5A4 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#5A4 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2
MALV-30A-Clone-6C2 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6C2 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7
MALV-30A-Clone-6D7 1.0 mg
EUR 448
Description:

Anti-Avian Leukosis Virus Antibody | MALV-30A-Clone#6D7 | Immunology Consultants Laboratory

Host: Mouse

Format: Unconjugated AP

Product Type: Primary Antibody

Antibody Clonality: Monoclonal

Human WDR78 shRNA Plasmid
20-abx962588
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse WDR78 shRNA Plasmid
20-abx982585
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Rat WDR78 shRNA Plasmid
20-abx989767
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Genorise® Sheep IL-6 cDNA Clone, 10 ug
GR151001 10 µg
EUR 183.6

Genorise® Ovine IL-2 cDNA Clone, 10 ug
GR151002 10 µg
EUR 183.6

Genorise® Ovine IL-4 cDNA Clone, 10 ug
GR151003 10 µg
EUR 183.6

Genorise® Bovine IL-1b cDNA Clone, 10 ug
GR152001 10 µg
EUR 183.6

Genorise® Rabbit IL-1b cDNA Clone, 10 ug
GR153001 10 µg
EUR 183.6

Genorise® Human H-FABP cDNA Clone, 10 ug
GR154001 10 µg
EUR 183.6

Genorise® Human Lp-PLA2 cDNA Clone, 10 ug
GR154007 10 µg
EUR 183.6

Genorise® Human PAPP-A cDNA Clone, 10 ug
GR154013 10 µg
EUR 183.6

Genorise® Human NT-proBNP cDNA Clone, 10 ug
GR154014 10 µg
EUR 183.6

Genorise® Human Pro-C3 cDNA Clone, 10 ug
GR154015 10 µg
EUR 183.6

Genorise® Canine NGF-B cDNA Clone, 10 ug
GR159001 10 µg
EUR 183.6

Genorise® Canine IL-7 cDNA Clone, 10 ug
GR159002 10 µg
EUR 183.6

Wdr78 ORF Vector (Rat) (pORF)
ORF079111 1.0 ug DNA
EUR 607.2

Wdr78 ORF Vector (Mouse) (pORF)
ORF061805 1.0 ug DNA
EUR 607.2

WDR78 ORF Vector (Human) (pORF)
ORF014948 1.0 ug DNA
EUR 424.8

WDR78 ORF Vector (Human) (pORF)
ORF011578 1.0 ug DNA
EUR 114

cDNA Synthesis SuperMix
20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100
EUR 424.8

Novo? cDNA Kit
M1165-25
EUR 320.4

Evo? cDNA Supermix
M1168-100
EUR 457.2

Evo? cDNA Supermix
M1168-25
EUR 320.4

Novo? cDNA Supermix
M1169-100
EUR 529.2

Novo? cDNA Supermix
M1169-25
EUR 346.8

Wdr78 sgRNA CRISPR Lentivector set (Mouse)
K4526301 3 x 1.0 ug
EUR 406.8

Wdr78 sgRNA CRISPR Lentivector set (Rat)
K6359901 3 x 1.0 ug
EUR 406.8

WDR78 sgRNA CRISPR Lentivector set (Human)
K2635401 3 x 1.0 ug
EUR 406.8

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

WD Repeat-Containing Protein 78 (WDR78) Antibody
abx037278-100ug 100 ug
EUR 469.2

Wdr78 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4526302 1.0 ug DNA
EUR 184.8

Wdr78 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4526303 1.0 ug DNA
EUR 184.8

Wdr78 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4526304 1.0 ug DNA
EUR 184.8

Wdr78 sgRNA CRISPR Lentivector (Rat) (Target 1)
K6359902 1.0 ug DNA
EUR 184.8

Wdr78 sgRNA CRISPR Lentivector (Rat) (Target 2)
K6359903 1.0 ug DNA
EUR 184.8

Wdr78 sgRNA CRISPR Lentivector (Rat) (Target 3)
K6359904 1.0 ug DNA
EUR 184.8

WDR78 sgRNA CRISPR Lentivector (Human) (Target 1)
K2635402 1.0 ug DNA
EUR 184.8

WDR78 sgRNA CRISPR Lentivector (Human) (Target 2)
K2635403 1.0 ug DNA
EUR 184.8

WDR78 sgRNA CRISPR Lentivector (Human) (Target 3)
K2635404 1.0 ug DNA
EUR 184.8

WDR78 Protein Vector (Human) (pPB-C-His)
PV046309 500 ng
EUR 394.8

WDR78 Protein Vector (Human) (pPB-N-His)
PV046310 500 ng
EUR 394.8

WDR78 Protein Vector (Human) (pPM-C-HA)
PV046311 500 ng
EUR 394.8

WDR78 Protein Vector (Human) (pPM-C-His)
PV046312 500 ng
EUR 394.8

WDR78 Protein Vector (Human) (pPB-C-His)
PV059789 500 ng
EUR 577.2

WDR78 Protein Vector (Human) (pPB-N-His)
PV059790 500 ng
EUR 577.2

WDR78 Protein Vector (Human) (pPM-C-HA)
PV059791 500 ng
EUR 577.2

WDR78 Protein Vector (Human) (pPM-C-His)
PV059792 500 ng
EUR 577.2

WDR78 Protein Vector (Mouse) (pPB-C-His)
PV247218 500 ng
EUR 1278

WDR78 Protein Vector (Mouse) (pPB-N-His)
PV247219 500 ng
EUR 1278

WDR78 Protein Vector (Mouse) (pPM-C-HA)
PV247220 500 ng
EUR 1278

WDR78 Protein Vector (Mouse) (pPM-C-His)
PV247221 500 ng
EUR 1278

WDR78 3'UTR GFP Stable Cell Line
TU078449 1.0 ml
EUR 1672.8

Wdr78 3'UTR GFP Stable Cell Line
TU273372 1.0 ml Ask for price

WDR78 3'UTR Luciferase Stable Cell Line
TU028449 1.0 ml
EUR 1672.8

Wdr78 3'UTR GFP Stable Cell Line
TU172263 1.0 ml Ask for price

Wdr78 3'UTR Luciferase Stable Cell Line
TU223372 1.0 ml Ask for price

Wdr78 3'UTR Luciferase Stable Cell Line
TU122263 1.0 ml Ask for price

WDR78 Protein Vector (Rat) (pPB-C-His)
PV316442 500 ng
EUR 1399.2

WDR78 Protein Vector (Rat) (pPB-N-His)
PV316443 500 ng
EUR 1399.2

WDR78 Protein Vector (Rat) (pPM-C-HA)
PV316444 500 ng
EUR 1399.2

WDR78 Protein Vector (Rat) (pPM-C-His)
PV316445 500 ng
EUR 1399.2

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit
BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit
BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution
AR0063 5mL
EUR 127.2

cDNA from Arteriosclerosis: Aorta
C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery
C1236013Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery
C1236013Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein
C1236020Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon
C1236090Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart
C1236122Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart
C1236122Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney
C1236142Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney
C1236142Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver
C1236149Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung
C1236152Ld-1 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung
C1236152Ld-2 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung
C1236152Ld-3 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung
C1236152Ld-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung
C1236152Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas
C1236188Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen
C1236246Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach
C1236248Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe
eNOS51-D-2 2 ug
EUR 534

Evo™ cDNA Kit
M1164-100 Ask for price

Evo™ cDNA Kit
M1164-25 Ask for price

Novo? Transcriptome cDNA Kit
M1167-100
EUR 1142.4

Novo? Transcriptome cDNA Kit
M1167-25
EUR 529.2

OneScriptPlus cDNA Synthesis Kit
G235 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis Kit
G236 100 x 20 ul reactions
EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix
G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454 100 x 20 ul reactions
EUR 202.8

circRNA cDNA Synthesis Kit
G627 25 rxn (20 ul/rxn)
EUR 370.8

Plant Tissue cDNA: Arabidopsis
PC34-310 10 rxn
EUR 498

WDR78 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV)
LV702027 1.0 ug DNA
EUR 540

WDR78 Lentiviral Vector (Human) (UbC) (pLenti-GIII-UbC)
LV702031 1.0 ug DNA
EUR 540

WDR78 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a)
LV702032 1.0 ug DNA
EUR 540

WDR78 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV664135 1.0 ug DNA
EUR 1626

WDR78 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV664139 1.0 ug DNA
EUR 1626

WDR78 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV664140 1.0 ug DNA
EUR 1626

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems
  • EUR 828.00
  • EUR 678.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Alzheimer's Disease: Brain
C1236035Alz 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain
C1236035Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain
C1236035Par 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus
C1236052Dem 40 reactions
EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus
C1236052Dep 40 reactions
EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon
C1236090Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus
C1236106Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart
C1236122Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum
C1236130Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney
C1236142Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver
C1236149Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung
C1236152Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung
C1236152Ld-5 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm
C1236169Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas
C1236188Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin
C1236218Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine
C1236226Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord
C1236234Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen
C1236246Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) cDNA Tissue: Thyroid
MR34-265 10 rxn
EUR 498

Evo? cDNA Kit (gDNA Removal)
M1166-100
EUR 457.2

Human Adult cDNA Tissue: Lung
HA-152 10 rxn
EUR 498

Human Adult cDNA Tissue: Skin
HA-218 10 rxn
EUR 498

Human Adult cDNA Tissue: Testis
HA-260 10 rxn
EUR 498

Human Tumor Tissue: Breast cDNA
HT05-090 10 rxn
EUR 498

Total-Transcriptome cDNA Synthesis Kit
G904 25 reactions
EUR 268.8

Total-Transcriptome cDNA Synthesis Kit
G905 100 reactions
EUR 652.8

Mouse iNOS (macrophage) cDNA probe
iNOS61-D-2 2 ug
EUR 534

Accuris qMax cDNA Synthesis Kit
PR2100-C-100 1 PC
EUR 405.3

Accuris qMax cDNA Synthesis Kit
PR2100-C-25 1 PC
EUR 170.83

Accuris qMax cDNA Synthesis Kit
PR2100-C-250 1 PC
EUR 854.12

Accuris qMax cDNA Synthesis Kit
PR2100-C-S 1 PC
EUR 93.31

amfiRivert cDNA Synthesis Master Mix
R5101-050 50 rxns
EUR 498

amfiRivert cDNA Synthesis Master Mix
R5101-100 2X50 rxns
EUR 1016.4

amfiRivert cDNA Synthesis Master Mix
R5101-200 4X50 rxns
EUR 1453.2

amfiRivet cDNA Synthesis 2X Buffer
R5102-050 500ul
EUR 160.8

amfiRivet cDNA Synthesis 2X Buffer
R5102-100 2x500ul
EUR 230.4

Human WD repeat- containing protein 78, WDR78 ELISA KIT
ELI-28748h 96 Tests
EUR 988.8

pCDH-CMV-MCS-EF1-Puro cDNA Vector cDNA Clon/Exp Vector [pre-packaged virus]
CD510VB-1 >1 x 10^6 IFUs
EUR 943.2

miRNA First-Strand cDNA Synthesis SuperMix
abx098020-20rxns20ulSystems 20 rxns × 20 ul Systems
EUR 878.4

Fly First-Strand cDNA Synthesis SuperMix
20-abx09885820ulSystems
  • EUR 861.60
  • EUR 693.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

All-in-One cDNA Synthesis SuperMix
B24403 200 ractions
EUR 547.2
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

All-in-One cDNA Synthesis SuperMix
B24408 1000 ractions
EUR 1743.6
Description: All-in-One cDNA Synthesis SuperMix contains all the necessary components pre-blended in one tube for reverse transcription.

AzuraQuant cDNA Synthesis Kit - 25 Reactions
AZ-1995 25 Reactions
EUR 183.6

AzuraQuant cDNA Synthesis Kit - 100 Reactions
AZ-1996 100 Reactions
EUR 446.4

cDNA from Human Tumor Tissue: Adrenal
C1235004-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bladder
C1235010-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Bone
C1235023-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Brain
C1235035-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Breast
C1235086 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Colon
C1235090 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Kidney
C1235142 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Liver
C1235149 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lung
C1235152 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Lymphoma
C1235161-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Mesenchymoma
C1235164-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Nose
C1235178-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Ovary
C1235183-10 10 reactions
EUR 592.8
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Pancreas
C1235188-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Parathyroid
C1235189-10 10 reactions
EUR 592.8
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Prostate
C1235201-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Rectum
C1235206-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Skin
C1235218-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Melanoma
C1235218A-10 10 reactions
EUR 338.4
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Stomach
C1235248 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Tumor Tissue: Uterus
C1235274 40 reactions
EUR 648
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Diabetic Tissue: Brain
C1236035Dia 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
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