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Extraction of high-quality tissue-specific RNA from London aircraft timber

November 6, 2020
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Extraction of high-quality tissue-specific RNA from London aircraft timber (Platanusacerifolia), allowing the development of a feminine inflorescence cDNA library

 

  • The London aircraft tree (PlatanusacerifoliaWilld.) has international significance as an city landscaping tree and is the topic of genetic-improvement applications for productive sterility, illness and/or insect resistance. Molecular evaluation methods are essential to such applications, however could also be impeded by particular difficulties encountered throughout nucleic acid isolation.

 

  • An in depth RNA isolation and purification protocol, primarily based on established cetyltrimethyl-ammonium bromide (CTAB) extraction methods mixed with further purification steps utilizing butanol and the ionic detergent CTAB, which overcomes these issues within the woody species P. acerifolia, was carried out. Briefly, phenolic compounds are sure to soluble polyvinylpyrrolidone after which separated out by way of LiCl precipitation of the RNA.

 

  • Subsequently, protein- and carbohydrate-contaminants are eliminated by chloroform partitioning adopted by LiCl-mediated precipitation. The ensuing isolates of RNA have been discovered to be of enough high quality for profitable use in reverse transcription PCR evaluation.

 

  • Moreover, RNA isolates from feminine inflorescences have been used for the development of a cDNA library. This library was discovered to include a number of full-length cDNA clones of MADS-box genes, according to the library being consultant of inflorescence expression profiles.
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LILRB2 Antibody

ABD9604 Lifescience Market 100 ug
EUR 438

LILRB2 Antibody

DF9604 Affbiotech 200ul
EUR 304
Description: LILRB2 Antibody detects endogenous levels of total LILRB2.

anti-LILRB2

YF-PA16864 Abfrontier 50 ul
EUR 363
Description: Mouse polyclonal to LILRB2

anti-LILRB2

YF-PA16865 Abfrontier 50 ug
EUR 363
Description: Mouse polyclonal to LILRB2

anti-LILRB2

YF-PA16866 Abfrontier 100 ug
EUR 403
Description: Rabbit polyclonal to LILRB2

LILRB2 cloning plasmid

CSB-CL839793HU-10ug Cusabio 10ug
EUR 612
Description: A cloning plasmid for the LILRB2 gene.

Anti-LILRB2 antibody

STJ112174 St John's Laboratory 100 µl
EUR 277
Description: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-LILRB2 antibody

STJ114050 St John's Laboratory 100 µl
EUR 277
Description: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

LILRB2 Rabbit pAb

A12157-100ul Abclonal 100 ul
EUR 308

LILRB2 Rabbit pAb

A12157-200ul Abclonal 200 ul
EUR 459

LILRB2 Rabbit pAb

A12157-20ul Abclonal 20 ul
EUR 183

LILRB2 Rabbit pAb

A12157-50ul Abclonal 50 ul
EUR 223

LILRB2 Rabbit pAb

A10135-100ul Abclonal 100 ul
EUR 308

LILRB2 Rabbit pAb

A10135-200ul Abclonal 200 ul
EUR 459

LILRB2 Rabbit pAb

A10135-20ul Abclonal 20 ul
EUR 183

LILRB2 Rabbit pAb

A10135-50ul Abclonal 50 ul
EUR 223

LILRB2 Conjugated Antibody

C35791 SAB 100ul
EUR 397

LILRB2 Blocking Peptide

DF9604-BP Affbiotech 1mg
EUR 195

pDONR223-LILRB2 Plasmid

PVTB00316 Lifescience Market 2 ug
EUR 356

Anti-LILRB2 (1D4)

YF-MA17235 Abfrontier 100 ug
EUR 363
Description: Mouse monoclonal to LILRB2

LILRB2 Recombinant Protein

96-515 ProSci 0.1 mg
EUR 516.5
Description: Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

LILRB2 Recombinant Protein

96-915 ProSci 0.1 mg
EUR 516.5
Description: Leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) is also known as CD85 antigen-like family member D (CD85d), Immunoglobulin-like transcript 4 (ILT-4), Monocyte / macrophage immunoglobulin-like receptor 10 (MIR-10), which is a member of the the subfamily B class of LIR receptors. LILRB2 is receptor for class I MHC antigens. LILRB2 recognizes a broad spectrum of HLA-A, HLA-B, HLA-C and HLA-G alleles. LILRB2 competes with CD8A for binding to class I MHC antigens. LILRB2 / CD85d inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions.

LILRB2 Recombinant Protein

91-439 ProSci 0.05 mg
EUR 448.25
Description: Members of the immunoglobulin-like transcript (ILT) family are activating and inhibitory immunoreceptors whose genes are located same locus that encodes killer cell Ig-like receptors (KIR). Leukocyte Immunoglobulin-Like Receptor Subfamily B Member 2 (LIR-2) is a type I transmembrane protein. LIR-2 is expressed primarily on monocytes and dendritic cells (DC). Human LIR-2 is produced as a 598 amino acino acid precursor including a 21 aa signal sequence, a 440 aa extracellular domain (ECD), a 21 aa transmenbrane segment, and a 116 aa cytoplasmic domain. LIR-2 binds to Classical MHCI proteins. Ligation of LIR-2 incluces Tyr phosphorylation within its cytoplasmic ITIMs, a requirement for association with SHP-1. LIR-2 mediates tolerogenic DC-induced CD4+ T cell energy in vitro and in vivo.

LILRB2 Antibody, HRP conjugated

1-CSB-PA013000LB01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

LILRB2 Antibody, FITC conjugated

1-CSB-PA013000LC01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

LILRB2 Antibody, Biotin conjugated

1-CSB-PA013000LD01HU Cusabio
  • EUR 317.00
  • EUR 335.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against LILRB2. Recognizes LILRB2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Human LILRB2 shRNA Plasmid

20-abx956969 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

LILRB2 Polyclonal Conjugated Antibody

C41960 SAB 100ul
EUR 397

Human LILRB2 ELISA KIT

ELI-27802h Lifescience Market 96 Tests
EUR 824

LILRB2 ELISA KIT|Human

EF006426 Lifescience Market 96 Tests
EUR 689

Human LILRB2 ELISA Kit

ELA-E7745h Lifescience Market 96 Tests
EUR 824

LILRB2 Recombinant Protein (Human)

RP017797 ABM 100 ug Ask for price

LILRB2 ELISA Kit (Human) (OKEH01827)

OKEH01827 Aviva Systems Biology 96 Wells
EUR 727
Description: Description of target: This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.1 ng/mL

LILRB2 ORF Vector (Human) (pORF)

ORF005933 ABM 1.0 ug DNA
EUR 95

Human CellExp? LILRB2, human recombinant

P1147-10 Biovision
EUR 131

Human CellExp? LILRB2, human recombinant

P1147-50 Biovision
EUR 403

Recombinant Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2)

4-RPB153Hu01 Cloud-Clone
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 expressed in: E.coli

Recombinant Human LILRB2/CD85d/ILT4 Protein

RP00262 Abclonal 10 μg
EUR 149

Polyclonal LILRB2 antibody - C-terminal region

APR01570G Leading Biology 0.05mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human LILRB2 - C-terminal region. This antibody is tested and proven to work in the following applications:

LILRB2 sgRNA CRISPR Lentivector set (Human)

K1215401 ABM 3 x 1.0 ug
EUR 339

cDNA Synthesis SuperMix

20-abx09801420ulSystems Abbexa
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100 Biovision
EUR 354

Novo? cDNA Kit

M1165-25 Biovision
EUR 267

Evo? cDNA Supermix

M1168-100 Biovision
EUR 381

Evo? cDNA Supermix

M1168-25 Biovision
EUR 267

Novo? cDNA Supermix

M1169-100 Biovision
EUR 441

Novo? cDNA Supermix

M1169-25 Biovision
EUR 289

LILRB2 sgRNA CRISPR Lentivector (Human) (Target 1)

K1215402 ABM 1.0 ug DNA
EUR 154

LILRB2 sgRNA CRISPR Lentivector (Human) (Target 2)

K1215403 ABM 1.0 ug DNA
EUR 154

LILRB2 sgRNA CRISPR Lentivector (Human) (Target 3)

K1215404 ABM 1.0 ug DNA
EUR 154

LILRB2 Protein Vector (Human) (pPB-C-His)

PV023729 ABM 500 ng
EUR 329

LILRB2 Protein Vector (Human) (pPB-N-His)

PV023730 ABM 500 ng
EUR 329

LILRB2 Protein Vector (Human) (pPM-C-HA)

PV023731 ABM 500 ng
EUR 329

LILRB2 Protein Vector (Human) (pPM-C-His)

PV023732 ABM 500 ng
EUR 329

LILRB2 3'UTR GFP Stable Cell Line

TU062462 ABM 1.0 ml
EUR 1394

LILRB2 3'UTR Luciferase Stable Cell Line

TU012462 ABM 1.0 ml
EUR 1394

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse)

4-PAB153Hu01 Cloud-Clone
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2)

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), APC

4-PAB153Hu01-APC Cloud-Clone
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with APC.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), Biotinylated

4-PAB153Hu01-Biotin Cloud-Clone
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with Biotin.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), Cy3

4-PAB153Hu01-Cy3 Cloud-Clone
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with Cy3.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), FITC

4-PAB153Hu01-FITC Cloud-Clone
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with FITC.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), HRP

4-PAB153Hu01-HRP Cloud-Clone
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with HRP.

Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2) Polyclonal Antibody (Human, Mouse), PE

4-PAB153Hu01-PE Cloud-Clone
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against Human, Mouse Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 2 (LILRB2). This antibody is labeled with PE.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems Abbexa
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems Abbexa
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 Bioline 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 Bioline 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 Bioline 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Bioline Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 Bioline 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 BosterBio 5mL
EUR 106

Plant Tissue cDNA: Arabidopsis

PC34-310 Alpha Diagnostics 10 rxn
EUR 415

Human eNOS cDNA probe

eNOS51-D-2 Alpha Diagnostics 2 ug
EUR 445

OneScriptPlus cDNA Synthesis Kit

G235 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 ABM 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 ABM 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 ABM 25 rxn (20 ul/rxn)
EUR 309

Evo™ cDNA Kit

M1164-100 Biovision Ask for price

Evo™ cDNA Kit

M1164-25 Biovision Ask for price

Novo? Transcriptome cDNA Kit

M1167-100 Biovision
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25 Biovision
EUR 441

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
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Transcriptome evaluation of leaf tissue from Bermudagrass (Cynodondactylon) utilizing a normalised cDNA library

 

A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodondactylon L. A complete of 15 588 high-high quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to provide a unigene set.

 

A complete of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation process was efficient. A big-scale comparative genomic evaluation of the unigenes was carried out utilizing publicly obtainable instruments, reminiscent of BLAST, InterProScan and Gene Ontology. The unigenes have been additionally subjected to a seek for EST-derived easy sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), that are helpful as DNA markers.

 

Though the candidate EST-SSRs and CISPs discovered within the current examine should be empirically examined, they’re anticipated to be helpful as DNA markers for a lot of functions, together with comparative genomic research of grass species, by advantage of their vital similarities to EST sequences from different grasses. Thus, data of Cynodon ESTs will empower turfgrass analysis by offering homologues for genes which are thought to confer vital features in different crops.

 

Lengthy-read cDNA Sequencing Allows a ‘Gene-Like’ Transcript Annotation of Transposable Parts

 

  • Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single locus analysis. In distinction, transposable factor (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs prevents the power to tell apart between doubtlessly useful expressed parts and degraded copies.

 

  • To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana strains poor in a number of layers of TE repression. These uniquely-mapping transcripts have been used to establish the set of TEs capable of generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we have now layered upon the present high-quality neighborhood normal annotation.

 

  • We used this annotation to cut back the bioinformatic complexity related to multi-mapping reads from short-read RNA-seq experiments, and we present that this enchancment is expanded in a TE-rich genome reminiscent of maize. Our TE annotation additionally allows the testing of particular standing hypotheses within the TE discipline.

 

  • We display that incorrect TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work supplies a brand new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to cut back the bioinformatic complexity related to repetitive TEs in any organism.

Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat

 

Background: Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection.
The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats.
Methods: A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samples collected in our previous study of S. japonicum infection.
Both plasma and serum samples were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). In addition, 120 goat plasma samples from an S. japonicum-endemic area (Wangjiang) and 33 from a non-endemic region (Weihai) were collected and evaluated using our method.
Results: The sensitivity and specificity of the real-time PCR for detecting infected samples were 98.74% (157/159, 95% CI: 95.53-99.85%) and 100% (94/94, 95% CI: 96.15-100%), respectively. For the ELISA, sensitivity and specificity were 98.11% (156/159, 95% CI: 94.59-99.61%) and 90.43% (85/94, 95% CI: 82.60-95.53%), respectively. Further, we found positivity rates for S. japonicum infection in Wangjiang and Weihai of 8.33% (10/120, 95% CI: 4.07-14.79%) and 0% (0/33, 95% CI: 0-10.58%), respectively.
Conclusions: The results of this study indicate that our real-time PCR method exhibits higher sensitivity and specificity than ELISA and is a useful method for detection of S. japonicum infection in goats.

Use of the PCR in a Combined Methodological Approach for the Study of Human Fascioliasis in an Endemic Area

 

Purpose: Fascioliasis is a worldwide distributed trematodiasis considered a neglected disease. Diagnosis in humans has been traditionally based on parasitological and immunological techniques.
Recently we reported the use of the PCR in stool samples for the individual diagnosis. The purpose of this study was to evaluate human fascioliasis by a combination of diagnostic methods in an area where the disease is highly endemic in animals.
Methods: We studied all the inhabitants (N = 240) of Tatón village, Argentina, by Fasciola hepatica rproCL1-ELISA. Among them, we continued the study with 13 cases that had at least two positive serological tests, who performed a questionnaire, physical examination, abdominal ultrasonography, and collection of blood and faeces. Blood/serum samples were used for Fh rproCL1-ELISA and liver function tests. Faeces were used for parasitological analysis and PCR of a repetitive fragment of Fasciola sp.
Results: Among the 13 patients, 9 presented symptoms of biliary colic. All patients repeated positive serology. F. hepatica eggs were not detected. PCR was positive in 11 cases.
Conclusion: This is the first report employing an approach based on the combination of methods for the evaluation of human fascioliasis in an endemic area, which includes molecular tools with a high value in detecting low infections.

GTPBP8 cloning plasmid

CSB-CL818697HU1-10ug Cusabio 10ug
EUR 233
Description: A cloning plasmid for the GTPBP8 gene.

GTPBP8 cloning plasmid

CSB-CL818697HU2-10ug Cusabio 10ug
EUR 233
Description: A cloning plasmid for the GTPBP8 gene.

Anti-GTPBP8 antibody

STJ118642 St John's Laboratory 100 µl
EUR 277

GTPBP8 Polyclonal Antibody

29768-100ul SAB 100ul
EUR 252

GTPBP8 Polyclonal Antibody

29768-50ul SAB 50ul
EUR 187

GTPBP8 Rabbit pAb

A16189-100ul Abclonal 100 ul
EUR 308

GTPBP8 Rabbit pAb

A16189-200ul Abclonal 200 ul
EUR 459

GTPBP8 Rabbit pAb

A16189-20ul Abclonal 20 ul
EUR 183

GTPBP8 Rabbit pAb

A16189-50ul Abclonal 50 ul
EUR 223

Rat GTPBP8 shRNA Plasmid

20-abx990090 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Mouse GTPBP8 shRNA Plasmid

20-abx975375 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human GTPBP8 shRNA Plasmid

20-abx959177 Abbexa
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

GTPBP8 Polyclonal Conjugated Antibody

C29768 SAB 100ul
EUR 397

GTPBP8 Recombinant Protein (Rat)

RP203999 ABM 100 ug Ask for price

GTPBP8 Recombinant Protein (Human)

RP014239 ABM 100 ug Ask for price

GTPBP8 Recombinant Protein (Human)

RP014242 ABM 100 ug Ask for price

GTPBP8 Recombinant Protein (Mouse)

RP140480 ABM 100 ug Ask for price

GTPBP8 Recombinant Protein (Mouse)

RP140483 ABM 100 ug Ask for price

Gtpbp8 ORF Vector (Mouse) (pORF)

ORF046828 ABM 1.0 ug DNA
EUR 506

Gtpbp8 ORF Vector (Mouse) (pORF)

ORF046829 ABM 1.0 ug DNA
EUR 506

GTPBP8 ORF Vector (Human) (pORF)

ORF004747 ABM 1.0 ug DNA
EUR 95

GTPBP8 ORF Vector (Human) (pORF)

ORF004748 ABM 1.0 ug DNA
EUR 95

Gtpbp8 ORF Vector (Rat) (pORF)

ORF068001 ABM 1.0 ug DNA
EUR 506

GTPBP8 sgRNA CRISPR Lentivector set (Human)

K0919501 ABM 3 x 1.0 ug
EUR 339

Gtpbp8 sgRNA CRISPR Lentivector set (Mouse)

K4112501 ABM 3 x 1.0 ug
EUR 339

Gtpbp8 sgRNA CRISPR Lentivector set (Rat)

K7385901 ABM 3 x 1.0 ug
EUR 339

cDNA Synthesis SuperMix

20-abx09801420ulSystems Abbexa
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Novo? cDNA Kit

M1165-100 Biovision
EUR 354

Novo? cDNA Kit

M1165-25 Biovision
EUR 267

Evo? cDNA Supermix

M1168-100 Biovision
EUR 381

Evo? cDNA Supermix

M1168-25 Biovision
EUR 267

Novo? cDNA Supermix

M1169-100 Biovision
EUR 441

Novo? cDNA Supermix

M1169-25 Biovision
EUR 289

GTPBP8 sgRNA CRISPR Lentivector (Human) (Target 1)

K0919502 ABM 1.0 ug DNA
EUR 154

GTPBP8 sgRNA CRISPR Lentivector (Human) (Target 2)

K0919503 ABM 1.0 ug DNA
EUR 154

GTPBP8 sgRNA CRISPR Lentivector (Human) (Target 3)

K0919504 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4112502 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4112503 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4112504 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7385902 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7385903 ABM 1.0 ug DNA
EUR 154

Gtpbp8 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7385904 ABM 1.0 ug DNA
EUR 154

GTPBP8 Protein Vector (Human) (pPB-C-His)

PV018985 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPB-N-His)

PV018986 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPM-C-HA)

PV018987 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPM-C-His)

PV018988 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPB-C-His)

PV018989 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPB-N-His)

PV018990 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPM-C-HA)

PV018991 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Human) (pPM-C-His)

PV018992 ABM 500 ng
EUR 329

GTPBP8 Protein Vector (Rat) (pPB-C-His)

PV272002 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Rat) (pPB-N-His)

PV272003 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Rat) (pPM-C-HA)

PV272004 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Rat) (pPM-C-His)

PV272005 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPB-C-His)

PV187310 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPB-N-His)

PV187311 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPM-C-HA)

PV187312 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPM-C-His)

PV187313 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPB-C-His)

PV187314 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPB-N-His)

PV187315 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPM-C-HA)

PV187316 ABM 500 ng
EUR 603

GTPBP8 Protein Vector (Mouse) (pPM-C-His)

PV187317 ABM 500 ng
EUR 603

GTPBP8 3'UTR GFP Stable Cell Line

TU059450 ABM 1.0 ml
EUR 1394

GTPBP8 3'UTR Luciferase Stable Cell Line

TU009450 ABM 1.0 ml
EUR 1394

Gtpbp8 3'UTR GFP Stable Cell Line

TU255567 ABM 1.0 ml Ask for price

Gtpbp8 3'UTR Luciferase Stable Cell Line

TU205567 ABM 1.0 ml Ask for price

Gtpbp8 3'UTR GFP Stable Cell Line

TU159227 ABM 1.0 ml Ask for price

Gtpbp8 3'UTR Luciferase Stable Cell Line

TU109227 ABM 1.0 ml Ask for price

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems Abbexa
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems Abbexa
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 Biochain 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit

BIO-65042 Bioline 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 Bioline 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 Bioline 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Bioline Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 Bioline 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 BosterBio 5mL
EUR 106

Plant Tissue cDNA: Arabidopsis

PC34-310 Alpha Diagnostics 10 rxn
EUR 415

Human eNOS cDNA probe

eNOS51-D-2 Alpha Diagnostics 2 ug
EUR 445

OneScriptPlus cDNA Synthesis Kit

G235 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis Kit

G236 ABM 100 x 20 ul reactions
EUR 169

OneScriptPlus cDNA Synthesis SuperMix

G453 ABM 25 x 20 ul reactions
EUR 97

OneScriptPlus cDNA Synthesis SuperMix

G454 ABM 100 x 20 ul reactions
EUR 169

circRNA cDNA Synthesis Kit

G627 ABM 25 rxn (20 ul/rxn)
EUR 309

Evo™ cDNA Kit

M1164-100 Biovision Ask for price

Evo™ cDNA Kit

M1164-25 Biovision Ask for price

Novo? Transcriptome cDNA Kit

M1167-100 Biovision
EUR 952

Novo? Transcriptome cDNA Kit

M1167-25 Biovision
EUR 441

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 Biochain 40 reactions
EUR 811
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 Biochain 40 reactions
EUR 668
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.
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  • Extraction of high-quality tissue-specific RNA from London aircraft timber
  • Dissecting effectivity of a 5′ fast amplification of cDNA ends
  • Identification of mobile inhibitors in opposition to Chikungunya virus replication by a cDNA
  • In-Yeast Meeting of Coronavirus Infectious cDNA Clones
  • Identification of Avramr1 from Phytophthorainfestans utilizing lengthy learn and cDNA pathogen-enrichment
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